The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Results are shown in Table 1. 100 fmol 384-well (75 µL) 0. Prerequisites: SoftMax Pro 7. Step 2: Launch SoftMax Pro Software. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. microplates or in a cuvette at a. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. All stand-alone instrument functions can be controlled using the software. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. The plate. Sale. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. It can run samples based on pre-defined. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. 0 out of 5 $ 23,995 $ 22,495. Figure 3. If it looks fine, then proceed. Next generation SpectraMax® absorbance microplate readers. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. 58% for the detection module and 2. no. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. The guide also covers the software features, the specifications, and the accessories of the systems. Cell Cytotoxicity assay procedure. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. Products : General, Microplate Readers. Italic and boldface type are used for emphasis. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Find regional support. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. . 7°C (gradient heating design). For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. both cuvette-based and microplate reader applications on the same instrument. Most papers they cultured bacteria to OD600>0. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. We would like to show you a description here but the site won’t allow us. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. The screw remains inside the instrument. Ease of use. In this product video, see how the SpectraMax M5 quickly reads samples. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Positions H21-K22 contained plate blanks of PBS only. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. begin reading microplates with seven mouse clicks. Contact our support team with your questions regarding Molecular Devices products and applications. Endpoint, kinetic, spectrum. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. \ Figure 3. Find regional support. Selection of Water Constant in PathCheck settings. . 2. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. 1. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. The following document is intended to demonstrate setup of this instrument. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. An assay plate containing a 15-point standard curve was read on the SpectraMax. keeping with best practices, reader performance should. The temperature in the plate chamber is ambient and isothermal. No additional manuals are available. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. Figure 1. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. These lab workhorses are used in drug discovery, research, bioassay. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. We use Spectramax M5 microplate reader in our lab. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Please refer to the Obsolete Microplate Reader Models for more information. Cell Cytotoxicity assay procedure. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. Molecular Devices. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Molecular Devices: Molecular Devices. Maximum signal with lowest background. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Reporter Assay. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Table 1. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Fluorescence Microplate Readers. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Endpoint, kinetic, spectrum. Step 1: Turn on your microplate reader. 1. Add to Cart. Request a Quote!Introduction. Microplate Readers BioTek Epoch 2 Microplate Reader. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. The instrument uses a paired monochromator and xenon flash. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. Other features and benefits include: Eliminate changing out filters. SpectraMax i3x Multi-Mode Detection Platform. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. On the back, in the right hand top corner,. 1. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. In addition, the SpectraMax iD5 can be expanded to. Additionally, user-installable options allow the SpectraMax i3 System to. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. When the user applies a set temperature of 37°C, the top is heated to 37. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. For SpectraMax ABS Plus reader, r 2 = 0. Table 2 summarizes the results for the uniformity test. The SpectraMax M2 and M2e are versatile and reliable. Microplate reader assays requiring infrared (IR) or red-shifted. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. ATP standard curves read on SpectraMax microplate readers. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. 100 fmol 1536-well (8 µL) 0. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Access this by going to Help >. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. Italic and boldface type are used for emphasis. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. A preconfigured protocol is available in the software to. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. The calculated LOQ values were 0. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Figure 3. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. 306 plate air cal fail,low light. SpectraMax ® M2 Multi-Mode Microplate Reader. 0 out of 5 $ 9,995 $ 9,495. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. San Jose, Calif. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. 0 out of 5 $ 6,499. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 1. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. In addition, the SpectraMax® iD5 can be expanded to include bottom. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Nuclear Receptor. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). SpectraMax Spectrofluorometers. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. . Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. . Time-. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. Learn more about its features, specifications, and applications in this user. Selection of Water Constant in PathCheck settings. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Unlock your i3/i3x instrument after shipment. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. Step 4: Incubate at room temperature for 1 hour, protected from light. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. The University of Rouen in Normandy uses the SpectraMax iD3 and. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Published Dec 16, 2020 | Updated Dec 16, 2020. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. The system uses two scanning monochromators to determine the optimal excitation and emission settings. SpectraDrop High-Throughput Screening (HTS) Kit. . However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. For readers without a cuvette port, these options are not shown in the software. It features a modular design that allows users to add or change detection cartridges according to their needs. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. . The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 7°C and the bottom to 36. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. This unlocks the transport slide. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Wide range of applications The VersaMax reader covers. 2. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Fill a clean plate with water. SpectraMax Glo Steady-Luc Reporter Assay Kit. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. . The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. This unlocks the transport slide. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Five preferred detection modes. 6% for the on-board optics (monochromators). The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. The SpectraMax 190 reader can be integrated with Molecular . Assays can be better optimized by scanning across. 303 unable to cal dark current. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Temperature control. 305 reference level saturation. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 020 fmol 0. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). In . Measure more accurately. 304 signal level saturation. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. SpectraMax 250 User. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Microplate readers’ standard detection modes. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Contents of wells were pipetted up and down several times to mix. No accessories are needed and SoftMax® Pro Software. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The guide also covers the software features, the specifications, and the accessories of the systems. B: Selection of Excitation Wavelength. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax® ABS Plus Microplate Reader can run . The system uses two scanning monochromators to determine the optimal excitation and emission settings. Read More. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Compact and tunable absorbance microplate readers DATA SHEET. Blank subtraction and standard curve fitting were done automatically by the software. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Sensitive fluorescent quantitation of DNA with the Quant-iT. 2 only) SpectraMax M-series Microplate readers. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. SpectraMax Mini Plug-In for SoftMax Pro 7. We would like to show you a description here but the site won’t allow us. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). SpectraMax iD5 Multi-Mode Microplate Reader. Fully tested with validations, warranty. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 997 (n = 3). Open the top cover of the instrument. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. HTRF-compatible SpectraMax Multi-Mode Readers. The powerful analysis tools in the software generate a standard curve and calculated data. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The data suggests that there is little interference from dead cells when live cells are analyzed, however, there may be some interference from live cells when dead cells are analyzed. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. View the Molecular Devices website. 2 only: SpectraMax Mini Update. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. Summary of Contents for Molecular Devices SpectraMax M2. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. Figure 1. The chamber contains springs that position the cuvette in the proper alignment for a read. 40266 Email: drugdiscoverytech@lifetech. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Cell Analysis. 012 fmol 0. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 100 readings per well gives optimal Z’ values for this assay. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Our specialization servicing. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. View the Molecular Devices website. Our absorbance plate readers feature our PathCheck Sensor technology and. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. . . SpectraMax Spectrofluorometers. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. SpectraMax® Microplate Readers and SoftMax® Pro Software. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Wide range of applications. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Figure 6: Transcreener ADP2 FI assay principle. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 0 out of 5 $ 5,999. When the user applies a set temperature of 37°C, the top is heated to 37. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The plate reader can measure multiple readouts, including UV and visible absorbance. Absorbance (optical density, OD) was read at 570 nm and 605. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. flexibility of monochromator-based systems. Examples: “Press carefully to engage,” “ Do not. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. With optimized reagents, option to operate the. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 316 grating motor driver faulty. com. . The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Multimode Microplate Reader. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Temperature control. In . Multimode Microplate Readers. Reader modes are customized to your specific applications with options to upgrade easily. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2.